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Table 2 Four potential HetR targets chosen for further analysis and patA

From: Deep sequencing of HetR-bound DNA reveals novel HetR targets in Anabaenasp. strain PCC7120

Region location

RNA-seq RPKM fold changea

Gene function

Potential HetR binding site

WT 0 h/ΔhetR0 h

WT 6 h/ΔhetR6 h

WT 0 h/WT 6 h

WT 0 h/WT 12 h

WT 0 h/WT 21 h

  

patA (all0521)

-13

-8.0

-1.8

1.6

1.4

Pattern formation

TTGGCTCAAACCCAT

asr1469

-4.0b

1.7b

--Low coverage--

Small peptide

TTGGGTCTAACTTAT

asl2028

-2.5

-1.5

-3.7

1.2

1.4

Unknown

TCGGGGTAACAATCC

alr2242

1.3

-13.4

1.5

1.1

2.7

Possible NTPase

None found

alr3758

Low coverage

11 / 0c

12

3.9

7.7

Anti sigma factor antagonist

TTGGGTAAACCTGCT

  1. aWT RPKM data from Flaherty et al. [25].
  2. bLow coverage.
  3. c11 reads in WT at 6 h dropped to 0 in the ΔhetR mutant.