Figure 7
Growth inhibition and necrosis of seedlings caused by O -glycosylated proteins. Tobacco seedlings were treated for 9 days with culture media from the strains overexpressing the indicated O-glycosylated proteins and then assessed for necrosis and growth inhibition. A: Western-blot (anti-c-myc) showing the relative amounts of recombinant proteins in the culture media from the overexpressing strains. Medium from the wild-type strain (B05.10) and uninoculated medium (YGG-low) were used as controls. Each lane contained proteins precipitated from 1 ml of medium. Molecular weight makers are show to the left of each lane (kDa). B: Example seedlings treated with the culture media. C: Average weight (n=6) of treated seedling after the 9-day incubation. Different letters on bars indicate statistically significant differences with 0.99 confidence.