Figure 6
Deglycosylation of B. cinerea proteins with exo α1-2,3 and exo α1-6 mannosidases. Extracellular media from cultures of the strains overexpressing the indicated proteins were treated with the enzymes and analysed by western-blot with the indicated antibodies. SDS-PAGE of the untreated samples is also shown (Coomassie). The samples treated with the different enzymes, as well as the untreated controls, were identical and corresponded to 900 μl of culture medium. Arrows point to the positions in the Coomassie-stained gels that correspond to the bands observed in the western blots.