Figure 5

Ser/Thr-rich regions partially retain proteins with the mycelium. A: Fluorescence of transformants expressing a fusion proteins with BcSpl1, GFP, and with (BcSpl1-GFP-ST), or without (BcSpl1-GFP), a Ser/Thr-rich region from Cel5A. The wild-type strain B05.10 was used as control. B: SDS-PAGE and western blot (anti-GFP) comparing the amount of recombinant protein released to the culture medium (Med) and the amount associated with the mycelium (Myc) in cultures (16 hours in YGG-low medium) of the same strains as in (A). Amounts of proteins loaded were those contained in 1.125 ml of medium (1/67 of total in culture) or associated with 7.5 mg of mycelium (1/122 of total in culture). C: Percentages of recombinant protein released to the medium as soluble protein for the two strains, averaged for three independent cultures. Asterisk indicates a statistically significant difference between the two strains with 0.95 confidence (Mann-Whitney test).