Tetracycline effect on PAO1 GW colonization. LB cultures were inoculated at 108°CFU/mL. The cells were treated for 1 h with tetracycline at bacteriostatic concentration. Non-attached bacteria (FT, dark grey) and sessile bacteria (SC, grey) CFU were quantified after 20 min of incubation (see Materials and methods). Each point is the mean × SD of biological triplicates.