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Table 3 Differentially expressed miRNAs identified to be commonly presented in mouse lungs infected either with w81 or ma81 at 1 dpi

From: Differential microRNA expression following infection with a mouse-adapted, highly virulent avian H5N2 virus

miRNA

w81‡

ma81‡

1dpi

1dpi

Fold change*

P value§

Fold change

P value

let-7e-3p

2.6

0.008

2.5

0.048

miR-100-5p†

2.1

0.005

2

0.035

miR-130a-5p†

3.4

0.036

4.5

0.001

miR-146b-3p†

3.1

0.014

3.4

0.011

miR-147-3p†

2.7

0.013

7.8

0.045

miR-151-5p†

4.5

0.013

7.3

0.027

miR-155-3p†

2.3

0.015

6.2

0.024

miR-1843-3p

3.6

0.022

2.5

0.046

miR-190a-5p

−5

0.033

−3.1

0.045

miR-2145

2.5

0.017

3.5

0.024

miR-219-5p

−3.5

0.005

−2

0.039

miR-223-3p†

3.5

0.037

7.1

0.005

miR-299a-5p

2.3

0.004

2.1

0.018

miR-29b-3p

−3.6

0.012

−2.7

0.047

miR-301a-3p†

−6.9

0.019

−2.8

0.042

miR-30c-5p

2.5

0.038

2.6

0.035

miR-30e-5p

−4.3

0

−2.7

0.022

miR-328-3p

4.1

0.011

3.7

0.034

miR-33-5p

−6.5

0.024

−4.2

0.042

miR-3475-3p

2.8

0.019

2.3

0.011

miR-434-3p

2.3

0

3

0

miR-449a-5p

−3.2

0.016

−2.5

0.046

miR-495-3p†

2

0.042

2.4

0.039

miR-503-5p

−2.6

0.007

−2.6

0.029

miR-671-5p

−2.6

0.038

−3.5

0.017

miR-700-5p

2

0.003

2.6

0.026

miR-99b-5p

3.1

0.007

3.1

0.032

  1. ‡ global cellular miRNA expression patterns in mouse lungs infected either with w81 or ma81 were compared with those of uninfected control mouse lungs. Differentially expressed miRNAs with a p < 0.05 and fold change>2 are described.
  2. *Sequence reads were normalized to determine the number of transcripts per million (TPM) using the following formula: Normalized expression = Actual miRNA count/Total count of clean reads*1000000. Then, fold changes of miRNAs were evaluated using the following formula: Fold change = log2 (treatment/control).
  3. §P-value was calculated from the normalized expression values as previously described [66].
  4. °Commonly upregulated at both 1 and 3 dpi.