Bacterial species and plasmids | Bacterial strains and plasmid names | Relevant characteristics | Source or reference |
---|---|---|---|
R. equi | ATCC33701 | virulent strain | |
TKR255 | PvapA-lacZ fusion strain of ATCC33701 | This study | |
TKR303 | ΔvirS of TKR255 | This study | |
TKR474 | virRΔHTH of TKR255 | This study | |
E. coli | DH5α | F-, Φ80dlacZΔM15, Δ(lacZYA-rgF)U169, deoR, recA1, endA1, hsdR17(rK-, mK+), phoA, supE44, λ-, thi-1, gyrA96, relA1 | |
plasmid | pBluescript | Ampr | |
pTKR131 | pBluescript::aac(3)IV | This study | |
pECO101 | E. coli-C. jejuni shuttle vector | [23] | |
pTKR144 | pTKR131::oriT | This study | |
pTKR159 | pTKR144::PaphII | This study | |
pDelta | pTKR159::codA-upp | This study | |
pINT | pUC57::aac(3)IV-integrase | This study | |
pGEM-T Easy | Ampr | Promega | |
pTKR130 | pGEM, 3.5 kb fragment containg vapA | This study | |
pTKR139 | ΔvapA (codon4-189) of pTKR130 | This study | |
pTKR148 | pTKR139::lacZ | This study | |
pTKR169 | pDela::PvapA-lacZ | This study | |
pTKR333 | pGEM, 3.9 kb fragment containg virR | This study | |
pTKR223 | pGEM, 3.9 kb fragment containg virS | This study | |
pTKR361 | vapRΔHTH(codon2-50) of pTKR333 | This study | |
pTKR226 | ΔvirS (codon2-252) of pTKR223 | This study | |
pTKR265 | pDelta::vapRΔHTH | This study | |
pTKR391 | pDelta::ΔvirS | This study | |
pTKR174 | pGEM::PaphII | This study | |
pTKR340 | pGEM::PaphII-virS | This study | |
pTKR344 | pINT::PaphII-virS | This study | |
. | pTKR445 | pINT::PaphII-virSD57A | This study |
pTKR509 | pGEM::PvirR-VirR | This study | |
pTKR528 | pINT::PvirR-VirR | This study |