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Table 1 Bacteria and plasmids used in this study

From: VirS, an OmpR/PhoB subfamily response regulator, is required for activation of vapA gene expression in Rhodococcus equi

Bacterial species and plasmids

Bacterial strains and plasmid names

Relevant characteristics

Source or reference

R. equi

ATCC33701

virulent strain

 
 

TKR255

PvapA-lacZ fusion strain of ATCC33701

This study

 

TKR303

ΔvirS of TKR255

This study

 

TKR474

virRΔHTH of TKR255

This study

E. coli

DH5α

F-, Φ80dlacZΔM15, Δ(lacZYA-rgF)U169, deoR, recA1, endA1, hsdR17(rK-, mK+), phoA, supE44, λ-, thi-1, gyrA96, relA1

plasmid

pBluescript

Ampr

 
 

pTKR131

pBluescript::aac(3)IV

This study

 

pECO101

E. coli-C. jejuni shuttle vector

[23]

 

pTKR144

pTKR131::oriT

This study

 

pTKR159

pTKR144::PaphII

This study

 

pDelta

pTKR159::codA-upp

This study

 

pINT

pUC57::aac(3)IV-integrase

This study

 

pGEM-T Easy

Ampr

Promega

 

pTKR130

pGEM, 3.5 kb fragment containg vapA

This study

 

pTKR139

ΔvapA (codon4-189) of pTKR130

This study

 

pTKR148

pTKR139::lacZ

This study

 

pTKR169

pDela::PvapA-lacZ

This study

 

pTKR333

pGEM, 3.9 kb fragment containg virR

This study

 

pTKR223

pGEM, 3.9 kb fragment containg virS

This study

 

pTKR361

vapRΔHTH(codon2-50) of pTKR333

This study

 

pTKR226

ΔvirS (codon2-252) of pTKR223

This study

 

pTKR265

pDelta::vapRΔHTH

This study

 

pTKR391

pDelta::ΔvirS

This study

 

pTKR174

pGEM::PaphII

This study

 

pTKR340

pGEM::PaphII-virS

This study

 

pTKR344

pINT::PaphII-virS

This study

.

pTKR445

pINT::PaphII-virSD57A

This study

 

pTKR509

pGEM::PvirR-VirR

This study

 

pTKR528

pINT::PvirR-VirR

This study