Figure 2
Relationship of population dynamics of K. apiculata to intra/extra-cellular antifungal activity. Effect of the number of K. apiculata on antifungal compounds secretion was investigated by statistic correlation between the cells number and antifungal activity that was extracted from the cell-free culture (extracellular) and cells of K. apiculata (intracellular) respectively. The assays were performed in 50-mL BSM broth at 28°C with 1.0 × 108 cells/mL of K. apiculata initially. Samples were analyzed for the number of K. apiculata, extra- and intracellular activity at intervals of 2 h. Antifungal effects were recorded with inhibition diameter (mm) after 48 h culture at 28°C in PDA medium (2.0 × 105 spore/mL P. italicum spores).