Target gene | Primer sequence (5’-3’) | Direction | Amplicon size(bp) | PCR cyclic conditions | References |
---|---|---|---|---|---|
lmoO737 | AGG GCT TCA AGG ACT TAC CC | F | 691 | 94°C × 5'; (94°C × 30s, 54°C × 75′s, 72°C × 75′s)35; 72°C × 10' | [8] |
ACG ATT TCT GCT TGC CAT TC | R | ||||
lmo1118 | AGG GGT CTT AAA TCC TGG AA | F | 906 | Do | [8] |
CGG CTT GTT CGG CAT ACT TA | R | ||||
ORF2819 | AGC AAA ATG CCA AAA CTC GT | F | 471 | Do | [8] |
CAT CAC TAA AGC CTC CCA TTG | R | ||||
ORF2110 | AGT GGA CAA TTG ATT GGT GAA | F | 597 | Do | [8] |
CAT CCA TCC CTT ACT TTG GAC | R | ||||
Prs | GCT GAA GAG ATT GCG AAA GAA G | F | 370 | Do | [8] |
CAA AGA AAC CTT GGA TTT GCG G | R | ||||
inlA | ACG AGT AAC GGG ACA AAT GC | F | 800 | 94°C × 2'; (94°C × 20s, 55°C × 20s, 72°C × 50s)30; 72°C × 2' | [7] |
CCC GAC AGT GGT GCT AGA TT | R | ||||
inlC | AAT TCC CAC AGG ACA CAA CC | F | 517 | Do | [7] |
CGG GAA TGC AAT TTT TCA CTA | R | ||||
inlJ | TGT AAC CCC GCT TAC ACA GTT | F | 238 | Do | [7] |
AGC GGC TTG GCA GTC TAA TA | R | ||||
plcA | CTG CTT GAG CGT TCA TGT CTC ATC CCC C | F | 1484 | 95°C × 2'; (95°C × 15′s, 60°C × 30s, 72°C × 90s)35; 72°C × 10' | [60] |
CAT GGG TTT CAC TCT CCT TCT AC | R | ||||
prfA | CTG TTG GAG CTC TTC TTG GTG AAG CAA TCG | F | 1060 | Do | [60] |
AGC AAC CTC GGT ACC ATA TAC TAA CTC | R | ||||
actA | CGC CGC GGA AAT TAA AAA AAG A | F | 839 | Do | [61] |
ACG AAG GAA CCG GGC TGC TAG | R | ||||
hlyA | GCA GTT GCA AGC GCT TGG AGT GAA | F | 456 | Do | [62] |
GCA ACG TAT CCT CCA GAG TGA TCG | R | ||||
Iap | ACA AGC TGC ACC TGT TGC AG | F | 131 | Do | [63] |
TGA CAG CGT GTG TAG TAG CA | R | ||||
REP1R-I | IIIICGICGICATCIGGC | F | Several | 95°C × 7'; (95°C × 1', 44°C × 1', 65°C × 8')30; 65°C × 10' | [52] |
REP2-I | ICGICTTATCIGGCCTAC | R | |||
ERIC1R | ATGTAAGCTCCTGGGGATTCAC | F | Several | 95°C × 7'; (95°C × 1', 52°C × 1', 65°C × 8')30; 65°C × 10' | [64] |
ERIC2 | AAGTAAGTGACTGGGGTGAGCG | R |