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Table 2 Sequences and PCR cyclic conditions of primers used for detection of selected serogroups, virulence genes and molecular typing

From: Virulence and genotypic characterization of Listeria monocytogenes isolated from vegetable and soil samples

Target gene

Primer sequence (5’-3’)

Direction

Amplicon size(bp)

PCR cyclic conditions

References

lmoO737

AGG GCT TCA AGG ACT TAC CC

F

691

94°C × 5'; (94°C × 30s, 54°C × 75′s, 72°C × 75′s)35; 72°C × 10'

[8]

ACG ATT TCT GCT TGC CAT TC

R

lmo1118

AGG GGT CTT AAA TCC TGG AA

F

906

Do

[8]

CGG CTT GTT CGG CAT ACT TA

R

ORF2819

AGC AAA ATG CCA AAA CTC GT

F

471

Do

[8]

CAT CAC TAA AGC CTC CCA TTG

R

ORF2110

AGT GGA CAA TTG ATT GGT GAA

F

597

Do

[8]

CAT CCA TCC CTT ACT TTG GAC

R

Prs

GCT GAA GAG ATT GCG AAA GAA G

F

370

Do

[8]

CAA AGA AAC CTT GGA TTT GCG G

R

inlA

ACG AGT AAC GGG ACA AAT GC

F

800

94°C × 2'; (94°C × 20s, 55°C × 20s, 72°C × 50s)30; 72°C × 2'

[7]

CCC GAC AGT GGT GCT AGA TT

R

inlC

AAT TCC CAC AGG ACA CAA CC

F

517

Do

[7]

CGG GAA TGC AAT TTT TCA CTA

R

inlJ

TGT AAC CCC GCT TAC ACA GTT

F

238

Do

[7]

AGC GGC TTG GCA GTC TAA TA

R

plcA

CTG CTT GAG CGT TCA TGT CTC ATC CCC C

F

1484

95°C × 2'; (95°C × 15′s, 60°C × 30s, 72°C × 90s)35; 72°C × 10'

[60]

CAT GGG TTT CAC TCT CCT TCT AC

R

prfA

CTG TTG GAG CTC TTC TTG GTG AAG CAA TCG

F

1060

Do

[60]

AGC AAC CTC GGT ACC ATA TAC TAA CTC

R

actA

CGC CGC GGA AAT TAA AAA AAG A

F

839

Do

[61]

ACG AAG GAA CCG GGC TGC TAG

R

hlyA

GCA GTT GCA AGC GCT TGG AGT GAA

F

456

Do

[62]

GCA ACG TAT CCT CCA GAG TGA TCG

R

Iap

ACA AGC TGC ACC TGT TGC AG

F

131

Do

[63]

TGA CAG CGT GTG TAG TAG CA

R

REP1R-I

IIIICGICGICATCIGGC

F

Several

95°C × 7'; (95°C × 1', 44°C × 1', 65°C × 8')30; 65°C × 10'

[52]

REP2-I

ICGICTTATCIGGCCTAC

R

ERIC1R

ATGTAAGCTCCTGGGGATTCAC

F

Several

95°C × 7'; (95°C × 1', 52°C × 1', 65°C × 8')30; 65°C × 10'

[64]

ERIC2

AAGTAAGTGACTGGGGTGAGCG

R