Influence of alcohol treatment on intestinal permeability in conventional mice treated with experimental diets. The ovalbumin levels in the serum were determined as a measure of gut permeability (A). The IL-1β levels in the small intestine (B) were measured by ELISA. Liver damage score (C). The CV mice (control) had no hepatic lesions, the ethanol-fed CV mice presented diffuse hepatic microvacuolation, and the alcohol-fed CV mice that received a high-fiber diet exhibited discrete microvacuolation (D). H&E staining (200X). The data represent the mean ± SEM (n = 5-7/group). #p < 0.05, the conventional mice that received a low-fiber diet and underwent alcohol treatment (LF + Ethanol) vs. the conventional mice received a low-fiber diet and were not treated with alcohol (LF Control); ***p < 0.001, the conventional mice that were treated with alcohol and received a low-fiber diet (LF + Ethanol) vs. the conventional mice that were treated with alcohol and received a high-fiber diet (HF + Ethanol).