Differential interference contrast microscopy (DIC) and fluorescence microscopy with Nile Red staining of Leishmania amazonensis promastigotes without treatment (A, B) and treated with concentrations that corresponded to the IC
(C-F) and IC
(G-L) of BZTS for 48 h. In the treated promastigotes, the images suggest the concentration-dependent accumulation of lipid-storage bodies in the cytoplasm. (G, I, K) The parasites that were treated with the IC90 of BZTS were completely modified. Scale bar = 10 μm.