The oriT regions from RA3 of the IncU group and PromA plasmids. Alignment of DNA sequences of the mobCp/oriT region of RA3 ( [GenBank: DQ401103] coordinates 9703 – 9837 nt), pMRAD02 ([GenBank: CP001003.1]; coordinates 40344–40226) pSB102 ([GenBank: NC_003122]; coordinates 11373 – 11494 nt), pMOL98 ([GenBank; FJ 666348]; coordinates 45941 – 45808 nt), pIPO2 ([GenBank: NC_003213]; coordinates 11035 – 11167 nt) and pTer331 ([GenBank: EU 315244]; coordinates 10744 – 10877 nt). Identical nucleotides in all six plasmids are in white on black, those identical in three to five representatives shadowed grey. Putative promoter sequences and regulatory motifs are marked above the RA3 and below the PromA plasmid sequences. Black arrows indicate MobC binding site in RA3 identified in this work, grey arrows correspond to the arms of palindromes IR2 (GC-rich, conserved in PromA group) and IR3. Putative operators for auxiliary proteins in PromA plasmids are underlined and in italics. Start codons for homologs of MobC are in bold and italics.