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Table 1 Oligonucleotide probes and hybridization conditions used for CARD-FISH analysis of bacteria attached to LDPE fragments

From: Rapid bacterial colonization of low-density polyethylene microplastics in coastal sediment microcosms

Probe Nucleotide sequence(5′ -3′) Probe target %FAa °Cb References
NON338 ACT CCT ACG GGA GGC AGC Negative control 55 35 [34]
EUB338 I GCT GCC TCC CGT AGG AGT Most Bacteria 55 35 [35]
EUB338 II GCA GCC ACC CGT AGG TGT Planctomycetales 55 35 [36]
EUB338 III GCT GCC ACC CGT AGG TGT Verrumicrobiales 55 35 [36]
ARC94 TGC GCC ACT TAG CTG ACA Arcobacter 20 46 [37]
PSA184 CCC CTT TGG TCC GTA GAC Pseudoalteromonas, Colwellia 30 31 [38]
  1. aPer cent (v/v) formamide (FA) in hybridization buffer, based on [39] (NON338, EUB probes), [37] (ARC94) or [38] (PSA184).
  2. bHybridization temperature, based on [39] (NON338, EUB probes), [37] (ARC94) or a modification of [40] (PSA184).
  3. The fragments were retrieved from microcosms following 14 days of exposure to sediment from sampling site SP2 (Humber Estuary, UK).