Epifluorescence micrographs showing bacterial attachment onto LDPE microplastics. The micrographs were obtained following 14 days of exposure to coastal sediment (sampling site SP2) in experimental microcosms. Micrographs corresponding to staining by 4',6-diamidino-2-phenylindole (DAPI) are displayed in the left-hand panels (a, c and e). Micrographs corresponding to staining by CARD-FISH are displayed on the right-hand panels, as shown for the oligonucleotide probes NON338 (b), EUB338 I - III (d) and PSA184 (f). The scale bar is 20 μM.