Figure 3

Quantification of the infection of WT MEFs and Atg5 ^−/− MEFs with B. abortus -mCherry (A) or with B. melitensis- mCherry (B). MEFs were infected for 1h with Brucella-mCherry at an MOI of 300. Cells were observed by fluorescence microscopy at 6h, 9h, 12h, 18h and 24h p.i. Values represent the number of bacteria per infected cell as means ± SEM with n ≥ 50, where n is the number of observed infected cells. Statistical significance was calculated using the MannWhitney Rank Sum Test. # and ## indicate a significant difference with p <0.05 and p <0.01, respectively.