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Table 4 Quantification of bacterial cells at various concentrations in the presence of oxide nanoparticles

From: A comparison of conventional methods for the quantification of bacterial cells after exposure to metal oxide nanoparticles

Strain name

Control (No nanoparticles)a

ZnO (0.5 mg/ml)

TiO2(0.5 mg/ml)

SiO2(1 mg/ml)

FCM

OD660 b

FCM

OD660

FCM

OD660

FCM

OD660

Total cell no.

Live cell no.

Total cell no.

Live cell no.

Total cell no.

Live cell no.

 

Total cell no.

Live cell no.

 

S. enterica Newport

1.34 × 109

1.31 × 109

1.34 × 109

1.20 × 109

1.17 × 109

7.47 × 108

4.72 × 108

4.63 × 108

-

1.29 × 109

1.29 × 109

1.36 × 109

6.76 × 108

6.61 × 108

7.45 × 108

5.18 × 108

5.06 × 108

1.73 × 108

9.58 × 107

9.21 × 107

-

6.07 × 108

6.06 × 108

7.91 × 107

3.30 × 108

3.20 × 108

3.79 × 108

2.19 × 108

2.13 × 108

-c

7.78 × 107

7.34 × 107

-

3.04 × 108

3.03 × 108

4.47 × 108

1.51 × 108

1.47 × 108

1.96 × 108

8.89 × 107

8.77 × 107

-

6.56 × 107

6.21 × 107

-

1.19 × 108

1.18 × 108

2.87 × 108

1.18 × 108

1.14 × 108

1.50 × 108

7.51 × 107

7.37 × 107

-

6.01 × 107

5.68 × 107

-

1.00 × 108

9.99 × 107

1.73 × 108

S. epidermidis

3.43 × 108

3.38 × 108

3.43 × 108

1.65 × 107

1.50 × 107

1.59 × 108

3.06 × 107

3.03 × 107

-

1.75 × 108

1.73 × 108

3.96 × 108

1.73 × 108

1.70 × 108

1.59 × 108

4.37 × 107

3.66 × 107

1.19 × 108

6.91 × 107

6.89 × 107

-

1.57 × 108

1.55 × 108

1.59 × 108

8.41 × 107

2.96 × 107

6.67 × 107

3.67 × 107

2.94 × 107

5.32 × 107

5.34 × 107

5.30 × 107

-

7.56 × 107

7.42 × 107

7.96 × 107

4.10 × 107

1.87 × 107

2.69 × 107

2.14 × 107

1.63 × 107

3.98 × 107

2.88 × 107

2.85 × 107

-

3.57 × 107

3.48 × 107

2.69 × 107

4.04 × 107

1.48 × 107

1.37 × 107

1.74 × 107

1.32 × 107

2.69 × 107

3.27 × 107

3.25 × 107

0

3.99 × 107

3.87 × 107

2.69 × 107

E. faecalis

2.33 × 109

2.32 × 109

2.33 × 109

1.20 × 109

1.16 × 109

8.82 × 108

5.54 × 108

5.33 × 108

-

7.10 × 108

7.07 × 108

2.02 × 109

1.20 × 109

1.19 × 109

1.27 × 109

1.44 × 108

1.26 × 108

-

8.43 × 106

8.10 × 106

-

3.17 × 108

3.14 × 108

1.41 × 109

5.86 × 108

5.68 × 108

5.94 × 108

4.26 × 107

4.00 × 107

-

4.30 × 106

4.20 × 106

-

1.43 × 108

1.42 × 108

5.94 × 108

2.78 × 108

2.74 × 108

2.60 × 108

4.00 × 107

3.87 × 107

-

2.02 × 107

1.98 × 107

-

1.20 × 108

1.17 × 108

3.37 × 108

2.27 × 108

2.21 × 108

2.08 × 108

3.62 × 107

3.53 × 107

-

2.52 × 107

2.48 × 107

-

1.16 × 108

1.13 × 108

2.86 × 108

E. coli

6.04 × 108

5.57 × 108

6.04 × 108

8.96 × 107

7.17 × 107

2.94 × 108

1.69 × 107

1.50 × 107

-

2.17 × 108

2.04 × 108

5.51 × 108

2.98 × 108

2.76 × 108

3.21 × 108

6.04 × 107

4.17 × 107

9.85 × 107

4.89 × 107

4.39 × 107

-

2.07 × 108

1.93 × 108

3.38 × 108

1.51 × 108

1.41 × 108

1.52 × 108

4.80 × 107

3.42 × 107

-

5.99 × 107

5.11 × 107

-

1.38 × 108

1.23 × 108

1.87 × 108

6.55 × 107

6.02 × 107

6.34 × 107

3.75 × 107

2.51 × 107

-

5.12 × 107

4.20 × 107

-

6.31 × 107

5.55 × 107

8.11 × 107

5.47 × 107

5.20 × 107

3.68 × 107

3.28 × 107

1.87 × 107

-

4.47 × 107

4.07 × 107

-

5.10 × 107

4.44 × 107

8.11 × 107

  1. aBacterial cell number was measured by flow cytometry (FCM) and spectrophotometer method of optical density (OD) measurement after 1 hr exposure to ZnO, TiO2 and SiO2 nanoparticles; inoculum used for each experiment was indicated in the control samples, i.e. no nanoparticles.
  2. bPresented data were converted from each sample cells concentration according to the each species standard curve of cell/ml vs OD660 and as mean of triplicate with standard deviations (SD) of < 5%.
  3. cValue was negative.
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BMC Microbiology

ISSN: 1471-2180

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