Gene organization of the yraS locus and protein domains of the gene product. (A) The position of each gene and the direction of transcription are shown by arrows. The position and sequences of the yraS putative promoter (P), RBS, rho-independent transcriptional terminator (hairpin loop) are indicated. The flanking genes hp (hypothetical protein) and aadc (aromatic amino acid decarboxylase) are also shown. (B) Structural organization and domains of the YraS protein showing the COG2015 (light grey line) and the β-lactamase domain (red cassette). A blue mark inside the β-lactamase domain shows the position of a compositionally biased region not used in domain database search. (C) Sequences alignment of the Zn2+ binding domain from YraS homologue proteins. Amino acid sequences correspond to position 186 to 367. The amino acids involved in Zn2+ binding are underlined. Yras, Y. ruckeri; CCI04676, Microcystis aeruginosa; YP_006413521, Thiocystis violascens; NP_249431, P. aeruginosa; EGS71352, Vibrio cholerae; and AAA25989, Pseudomonas sp. ATCC19151.