Figure 6
From: Clostridium difficilehas a single sortase, SrtB, that can be inhibited by small-molecule inhibitors
SrtB ΔN26 cleaves FRET peptides between T and G residues. MALDI analysis of FRET reaction products revealed a fragment of mass 889.46, corresponding to the predicted mass of d-PVPPKT-OH (top) when d-PVPPKTGDS-e was incubated with SrtBΔN26. This fragment was absent in the mock treated peptide sample (bottom), indicating that SrtBΔN26 cleaves the d-PVPPKTGDS-e between the T and G residues.