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Table 6 A comparison of the expected and observed result, colour of colonies and sensitivity

From: Evaluation of the ability of four ESBL-screening media to detect ESBL-producing Salmonella and Shigella

 

ChromID ESBL

Brilliance ESBL

Drigalski (BLSE agar)

MacConkey (BLSE agar)

CHROMagar ESBL

Observed /Expected ESBL A -positive

51/51

51/51

50/51

50/51

51/51

Observed/Expected AmpC-positive

32/36

31/0

36/36

36/36

23/0

Expected colour of colonies

Colourless

Colourless

Blue

White

Colourless

Colour of Salmonella colonies

Colourless (n=62) Pink (n=3)

Colourless (n=61) Pink (n=3)

Blue

Pale pink

Colourless

Colour of Shigella sonnei colonies

Pink

Blue

Blue

Pale pink

Pink

Colour of Shigella flexneri colonies

Colourless

Colourless

Blue

Pale pink

Colourless

Sensitivity (95% CI*)

95% (90.4-99.6)

93% (87.6-98.4)

99% (96.9-100)

99% (96.9-100)

85% (77.5-92.5)

Sensitivity ESBL A (95% CI*)

100%

100%

98% (94.2-100)

98% (94.2-100)

100%

Sensitivity AmpC (95% CI*)

89% (78.8-99.2)

83% (70.7-95.3)

100%

100%

64% (48.3-79.7)

  1. A total of 87 ESBL-producing isolates (51=ESBLA, 36=AmpC) were inoculated on the four screening agars. BLSE agar is a biplate consisting of two different agars; Drigalski agar and MacConkey agar. The isolates were mixed with a fecal suspension before inoculation. The expected results are estimated by the manufacturers product information.
  2. *CI=95% Confidence interval.