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Table 1 Bacterial strains used in this work

From: Elevated recombinant clyA gene expression in the uropathogenic Escherichia coli strain 536, a clue to explain pathoadaptive mutations in a subset of extraintestinal E. coli strains

Strain Genotype/relevant characteristics, serotype Reference/source
ECOR collection E. coli reference collection [20]
536 Clinical UTI isolate, O6:K15:H31 [26]
J96 Clinical UTI isolate, O4:K6 [27]
JON47 J96 clyA + (Kmr) This work
JON53 536 clyA + (Kmr) This work
COE2 JON53 clyA-luxAB This work
COE3 COE3/pAES1 (sfaX + clone) This work
COE4 COE2/pBR322 This work
COE6 COE2/pHMG94 (papI + clone) This work
AD110 Clinical UTI isolate, O6:K2:H1:F7 [28]
DS-17 Clinical UTI isolate, O6:K5 [29]
IA-2 Clinical UTI isolate, O6:H [30]
IH11128 Clinical UTI isolate, O75:K5:H [31]
IHE3034 Clinical NBM isolate, O18:K1 [32]
AES1 sfaX::kan (Kmr) mutant of IHE3034 [33]
AES153 Δ sfaY mutant of IHE3034 This laboratory
DH5α endA1 hsdR17 (rkmk +) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF) [34]
MG1655 E. coli K-12 wildtype [35]
MC1061 araD 139 Δ(ara leu)7697 ΔlacX74galU galK hsr hsm + strA [36]
M182 Δ(lacIPOZY) X74 galK galU strA [36]
MC4100 araD139Δ(lac)U169 strA thi [37]
BSN26 MC4100 trp::tet (Tcr) [38]
JON33 BSN26 clyA::luxAB [8]
MWK11 MC4100 clyA ++ [8]
MWK7 M182 clyA This laboratory