Elevated recombinant clyA gene expression in the uropathogenic Escherichia coli strain 536, a clue to explain pathoadaptive mutations in a subset of extraintestinal E. coli strains

Table 1 Bacterial strains used in this work

Strain	Genotype/relevant characteristics, serotype	Reference/source
ECOR collection	E. coli reference collection	[20]
536	Clinical UTI isolate, O6:K15:H31	[26]
J96	Clinical UTI isolate, O4:K6	[27]
JON47	J96 clyA ⁺ (Km^r)	This work
JON53	536 clyA ⁺ (Km^r)	This work
COE2	JON53 clyA-luxAB	This work
COE3	COE3/pAES1 (sfaX ⁺ clone)	This work
COE4	COE2/pBR322	This work
COE6	COE2/pHMG94 (papI ⁺ clone)	This work
AD110	Clinical UTI isolate, O6:K2:H1:F7	[28]
DS-17	Clinical UTI isolate, O6:K5	[29]
IA-2	Clinical UTI isolate, O6:H	[30]
IH11128	Clinical UTI isolate, O75:K5:H	[31]
IHE3034	Clinical NBM isolate, O18:K1	[32]
AES1	sfaX::kan (Km^r) mutant of IHE3034	[33]
AES153	Δ sfaY mutant of IHE3034	This laboratory
DH5α	endA1 hsdR17 (r_km_k ⁺) supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)	[34]
MG1655	E. coli K-12 wildtype	[35]
MC1061	araD 139 Δ(ara leu)7697 ΔlacX74galU galK hsr hsm ⁺ strA	[36]
M182	Δ(lacIPOZY) X74 galK galU strA	[36]
MC4100	araD139Δ(lac)U169 strA thi	[37]
BSN26	MC4100 trp::tet (Tc^r)	[38]
JON33	BSN26 clyA::luxAB	[8]
MWK11	MC4100 clyA ⁺⁺	[8]
MWK7	M182 clyA	This laboratory

ISSN: 1471-2180