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Table 5 Fold change in gene expression along the cysteine and methionine metabolic pathway

From: Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain

Gene Product PM vs. WT 0 PM vs. WT 10 PM 0 vs. 10 PM 0 vs. 17.5 WT 0 vs. 10
   ML LL ML LL ML LL ML LL ML LL
Cthe_0290 homoserine dehydrogenase −1.03 1.21 2.33 1.94 −1.78 −1.38 1.35 1.17 1.45 −1.30
Cthe_0580 aminotransferase class I and II 1.22 1.48 −1.31 1.17 1.03 −1.00 1.44 2.03 1.64 1.26
Cthe_0715 S-adenosylmethionine decarboxylase proenzyme 1.21 1.33 2.95 −1.12 −1.51 −1.64 −1.87 −2.76 −3.67 −1.10
Cthe_0755 aminotransferase class I and II −2.42 −1.28 1.59 −1.40 −1.75 −1.37 −1.60 −2.06 −6.77 −1.25
Cthe_0961 aspartate-semialdehyde dehydrogenase −2.51 −2.11 −2.12 −1.37 1.18 1.15 1.47 2.34 −1.01 −1.34
Cthe_1053 L-lactate dehydrogenase −1.78 −1.25 1.32 −1.02 −1.41 −1.27 −1.33 −1.16 −3.30 −1.55
Cthe_1200 Adenosylhomocysteinase −1.26 1.07 2.23 1.76 1.39 1.18 1.01 −1.62 −2.02 −1.39
Cthe_1559 Cys/Met metabolism pyridoxal-phosphate-dependent protein −9.22 −5.72 −4.73 −3.97 4.66 3.12 16.05 8.31 2.39 2.17
Cthe_1560 Pyridoxal-5'-phosphate-dependent protein beta subunit −6.16 −2.97 −3.71 −2.65 6.25 3.41 15.55 6.43 3.77 3.05
Cthe_1569 Cys/Met metabolism pyridoxal-phosphate-dependent protein 1.02 1.09 −2.06 −1.83 3.94 2.46 5.21 4.42 8.24 4.90
Cthe_1728 DNA-cytosine methyltransferase 2.09 2.38 −1.21 2.26 1.03 −1.01 1.59 1.80 2.60 1.04
Cthe_1749 DNA-cytosine methyltransferase 1.08 −1.12 −5.98 −2.41 −1.08 1.20 1.13 1.46 5.95 2.58
Cthe_1840 cysteine synthase A −1.52 −1.21 3.14 2.17 1.37 1.27 1.83 −1.27 −3.48 −2.07
Cthe_1842 O-acetylhomoserine/O-acetylserine sulfhydrylase −1.68 −1.54 −1.10 1.52 1.51 1.16 2.46 1.75 −1.02 −2.01
  1. Bold values indicate significantly different levels of express as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.