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Table 5 Fold change in gene expression along the cysteine and methionine metabolic pathway

From: Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain

Gene

Product

PM vs. WT 0

PM vs. WT 10

PM 0 vs. 10

PM 0 vs. 17.5

WT 0 vs. 10

  

ML

LL

ML

LL

ML

LL

ML

LL

ML

LL

Cthe_0290

homoserine dehydrogenase

−1.03

1.21

2.33

1.94

−1.78

−1.38

1.35

1.17

1.45

−1.30

Cthe_0580

aminotransferase class I and II

1.22

1.48

−1.31

1.17

1.03

−1.00

1.44

2.03

1.64

1.26

Cthe_0715

S-adenosylmethionine decarboxylase proenzyme

1.21

1.33

2.95

−1.12

−1.51

−1.64

−1.87

−2.76

−3.67

−1.10

Cthe_0755

aminotransferase class I and II

−2.42

−1.28

1.59

−1.40

−1.75

−1.37

−1.60

−2.06

−6.77

−1.25

Cthe_0961

aspartate-semialdehyde dehydrogenase

−2.51

−2.11

−2.12

−1.37

1.18

1.15

1.47

2.34

−1.01

−1.34

Cthe_1053

L-lactate dehydrogenase

−1.78

−1.25

1.32

−1.02

−1.41

−1.27

−1.33

−1.16

−3.30

−1.55

Cthe_1200

Adenosylhomocysteinase

−1.26

1.07

2.23

1.76

1.39

1.18

1.01

−1.62

−2.02

−1.39

Cthe_1559

Cys/Met metabolism pyridoxal-phosphate-dependent protein

−9.22

−5.72

−4.73

−3.97

4.66

3.12

16.05

8.31

2.39

2.17

Cthe_1560

Pyridoxal-5'-phosphate-dependent protein beta subunit

−6.16

−2.97

−3.71

−2.65

6.25

3.41

15.55

6.43

3.77

3.05

Cthe_1569

Cys/Met metabolism pyridoxal-phosphate-dependent protein

1.02

1.09

−2.06

−1.83

3.94

2.46

5.21

4.42

8.24

4.90

Cthe_1728

DNA-cytosine methyltransferase

2.09

2.38

−1.21

2.26

1.03

−1.01

1.59

1.80

2.60

1.04

Cthe_1749

DNA-cytosine methyltransferase

1.08

−1.12

−5.98

−2.41

−1.08

1.20

1.13

1.46

5.95

2.58

Cthe_1840

cysteine synthase A

−1.52

−1.21

3.14

2.17

1.37

1.27

1.83

−1.27

−3.48

−2.07

Cthe_1842

O-acetylhomoserine/O-acetylserine sulfhydrylase

−1.68

−1.54

−1.10

1.52

1.51

1.16

2.46

1.75

−1.02

−2.01

  1. Bold values indicate significantly different levels of express as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.