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Table 4 Fold change in gene expression in histidine metabolism pathways

From: Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain

Gene Product PM vs. WT 0 PM vs. WT 10 PM 0 vs. 10 PM 0 vs. 17.5 WT 0 vs. 10
   ML LL ML LL ML LL ML LL ML LL
Cthe_2880 ATP phosphoribosyltransferase regulatory subunit 98.42 29.12 98.72 80.51 1.25 1.01 1.12 −1.06 1.25 −2.73
Cthe_2881 ATP phosphoribosyltransferase 78.48 23.79 85.06 100.15 1.64 1.24 1.35 −1.01 1.52 −3.40
Cthe_2882 histidinol dehydrogenase 35.86 18.44 28.45 44.69 1.49 1.33 1.37 1.40 1.88 −1.83
Cthe_2883 histidinol-phosphate aminotransferase 38.12 19.61 23.12 40.22 1.15 1.22 1.19 1.42 1.89 −1.69
Cthe_2884 Imidazoleglycerol-phosphate dehydratase 7.45 7.71 17.31 17.09 1.23 1.25 1.18 1.27 −1.89 −1.77
Cthe_2886 Imidazole glycerol phosphate synthase subunit hisH 11.99 12.29 14.84 15.87 1.19 1.12 1.09 −1.01 −1.04 −1.16
Cthe_2887 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase 13.46 11.01 10.02 14.54 1.44 1.20 1.29 1.13 1.93 −1.10
Cthe_2888 Imidazole glycerol phosphate synthase subunit hisF 12.46 14.23 10.04 18.19 1.61 1.30 1.54 1.24 1.99 1.02
Cthe_2889 Histidine biosynthesis bifunctional protein hisIE 12.71 10.80 6.09 12.49 1.48 1.29 1.51 1.28 3.08 1.11
Cthe_3028 Pyridoxal-dependent decarboxylase −11.35 −13.46 −7.10 −6.92 −2.37 −1.04 −3.78 −2.89 −3.79 −2.02
Cthe_3149 aminoacyl-histidine dipeptidase 3.34 4.23 −1.07 1.63 1.15 1.05 1.39 1.37 4.09 2.72
Cthe_1332 Histidyl-tRNA synthetase −1.58 −1.89 1.66 −1.18 1.10 −1.03 −1.15 −1.62 −2.38 −1.64
  1. Bold values indicate significantly different levels of expression as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.