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Table 3 Fold change in gene expression involved in cellular redox

From: Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain

  

PM vs. WT 0

PM vs. WT 10

PM 0 vs. 10

PM 0 vs. 17.5

WT 0 vs. 10

  

ML

LL

ML

LL

ML

LL

ML

LL

ML

LL

Redox transcriptional repressor

Cthe_0422

Redox-sensing transcriptional repressor rex

1.13

−1.08

7.01

5.53

1.04

−1.02

−1.04

−1.11

−5.96

−6.08

Ech-type hydrogenases

Cthe_3013

hydrogenase expression/formation protein HypE

1.39

1.19

3.42

2.34

−1.90

−2.24

1.30

−1.14

1.37

−1.03

Cthe_3016

[NiFe] hydrogenase maturation protein HypF

2.34

2.42

3.10

3.62

−1.03

−1.45

1.28

1.03

1.52

1.84

Cthe_3017

hydrogenase accessory protein HypB

2.66

3.07

2.56

3.73

1.12

−1.15

1.08

1.06

1.63

1.97

Cthe_3018

hydrogenase expression/synthesis HypA

2.51

3.11

2.20

3.99

1.40

−1.07

1.22

1.20

1.92

2.27

Cthe_3019

4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

2.89

3.12

1.77

2.98

1.14

1.03

1.54

2.02

1.87

1.08

Cthe_3020

NADH-ubiquinone oxidoreductase chain 49 kDa

2.96

3.83

1.86

3.15

1.02

−1.03

1.55

2.07

1.64

1.18

Cthe_3021

ech hydrogenase, subunit EchD, putative

4.29

4.79

2.15

3.03

−1.12

−1.09

1.16

1.71

1.79

1.46

Cthe_3024

NADH/Ubiquinone/plastoquinone (complex I)

2.04

2.26

2.83

2.10

−1.12

−1.10

−1.17

1.05

−1.54

−1.02

ATP synthase

Cthe_2602

ATP synthase subunit a

2.77

3.55

5.58

3.87

−1.21

1.10

−1.35

−1.72

−2.44

1.01

Cthe_2603

ATP synthase subunit c

2.27

2.68

2.66

4.62

1.11

1.04

−1.04

−1.22

−1.06

−1.66

Cthe_2604

ATP synthase subunit b

2.48

2.18

4.03

4.30

−1.01

1.10

−1.23

−1.32

−1.64

−1.80

Cthe_2605

ATP synthase F1, delta subunit

3.55

2.04

3.86

2.95

−1.06

−1.05

−1.77

−2.01

−1.15

−1.53

Cthe_2606

ATP synthase F1, alpha subunit

2.40

2.00

2.75

3.27

1.60

1.31

1.20

1.25

1.40

−1.24

Cthe_2607

ATP synthase F1, gamma subunit

2.63

2.09

2.06

2.95

1.17

1.20

1.09

1.49

1.50

−1.18

Cthe_2608

ATP synthase F1, beta subunit

2.67

2.65

3.73

4.36

1.40

1.37

1.04

1.05

−1.00

−1.20

Cthe_2609

ATP synthase epsilon chain

2.94

2.87

4.11

4.79

1.12

1.33

−1.21

−1.11

−1.24

−1.26

  1. Bold values indicate significantly different levels of expression as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.