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Table 2 Fold change in gene expression along the central metabolism and mixed-acid fermentation pathways

From: Transcriptomic analysis of Clostridium thermocellum Populus hydrolysate-tolerant mutant strain shows increased cellular efficiency in response to Populus hydrolysate compared to the wild type strain

Gene Product PM vs. WT 0 PM vs. WT 10 PM 0 vs. 10 PM 0 vs. 17.5 WT 0 vs. 10
   ML LL ML LL ML LL ML LL ML LL
glucose-6-phosphate to PEP
Cthe_0347 Phosphofructokinase 1.77 2.59 2.97 2.13 −1.35 −1.31 −1.14 −1.49 −2.27 −1.07
Cthe_0349 fructose-1,6-bisphosphate aldolase, class II 1.60 2.49 3.31 2.50 −1.52 −1.41 −1.49 −2.03 −3.14 −1.42
Cthe_2449 Phosphoglycerate mutase −2.46 −1.85 1.42 −1.74 −1.48 −1.90 −2.01 −2.88 −5.18 −2.03
Cthe_3153 alpha-ribazole phosphatase 2.11 2.33 1.40 1.40 1.21 1.23 1.42 −1.14 1.82 2.04
Cthe_0143 Enolase −1.23 −1.04 1.63 −1.02 −1.11 −1.13 −1.05 −2.96 −2.22 −1.16
Non-oxidative Pentose Phosphate pathway
Cthe_2443 Transketolase domain-containing protein −3.24 −4.70 1.02 −3.00 1.14 −1.75 −1.90 −1.52 −2.88 −2.74
Cthe_2444 Transketolase domain-containing protein −3.47 −4.63 −1.15 −3.39 1.26 −1.72 −1.63 −1.57 −2.41 −2.36
Cthe_2705 Transketolase central region −1.44 −1.33 2.25 1.19 1.24 1.21 1.17 −1.81 −2.60 −1.32
PEP to Pyruvate
Cthe_2874 Phosphoenolpyruvate carboxykinase [GTP] 1.39 2.46 1.43 2.09 −1.05 −1.04 1.30 1.38 −1.07 1.14
Cthe_0344 malic protein NAD-binding −1.68 1.06 1.26 −1.10 −1.01 −1.14 1.20 −1.27 −2.13 1.02
Cthe_1308 pyruvate, phosphate dikinase 1.64 2.29 −1.30 1.65 −1.05 1.07 1.30 1.10 2.03 1.49
Pyruvate to Lactate/Formate/Acetyl-CoA
Cthe_1053 L-lactate dehydrogenase −1.78 −1.25 1.32 −1.02 −1.41 −1.27 −1.33 −1.16 −3.30 −1.55
Cthe_2794 pyruvate/ketoisovalerate oxidoreductase, gamma subunit 4.30 1.48 5.15 3.99 1.92 2.56 2.45 2.78 1.61 −1.05
Cthe_2796 pyruvate flavodoxin/ferredoxin oxidoreductase domain protein 3.13 1.47 4.16 2.94 1.98 2.05 1.88 1.94 1.49 1.02
Cthe_0505 formate acetyltransferase −1.95 −1.91 1.46 −1.04 1.24 1.04 1.11 −1.81 −2.31 −1.76
Acetyl-CoA to Ethanol/Acetate
Cthe_1028 Acetate kinase 1.67 2.57 3.63 3.05 2.12 1.26 2.76 1.50 −1.02 1.06
Cthe_1029 phosphate acetyltransferase 1.54 1.79 4.01 3.83 2.42 1.33 2.73 1.63 −1.08 −1.61
Cthe_2238 Aldehyde Dehydrogenase 1.06 1.04 −1.81 −1.29 1.20 1.36 1.36 1.02 2.30 1.83
Cthe_0101 iron-containing alcohol dehydrogenase −1.35 −1.19 2.19 1.20 1.22 −1.04 −1.18 −1.82 −2.43 −1.48
Cthe_0423 iron-containing alcohol dehydrogenase 1.12 1.07 4.75 5.02 1.26 1.06 1.28 1.45 −3.36 −4.42
  1. Bold values indicate significantly different levels of expression as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.