Table 2 Protein names, putative function, and % identity of the encoded Hpi, Amb and Wel enzymes
Enzyme | FS ATCC 43239 | FS PCC 9339 | FA UTEX 1903 | HW IC-52-3 | WI HT-29-1 | FS PCC 9431 | FM SAG 1427-1 | % identity* |
|---|---|---|---|---|---|---|---|---|
Tryptophan biosynthesis: | Â | Â | Â | Â | Â | Â | Â | Â |
TrpE | HpiT1 | HpiT1 | AmbT1 | WelT1 | WelT1 | WelT1 | WelT1 | 93.3 |
TrpC | HpiT2 | HpiT2 | AmbT2 | WelT2 | WelT2 | WelT2 | WelT2 | 92 |
TrpA | HpiT3 | HpiT3 | AmbT3 | WelT3 | WelT3 | WelT3 | WelT3 | 92.7 |
TrpB | HpiT4 | HpiT4 | AmbT4 | WelT4 | WelT4 | WelT4 | WelT4 | 95.7 |
TrpD | HpiT5 | HpiT5 | AmbT5 | WelT5 | WelT5 | WelT5 | WelT5 | 94.8 |
DAHP synthase | HpiC2 | HpiC2 | AmbC2 | WelC2 | WelC2 | WelC2 | WelC2 | 95.3 |
IPP and DMAPP biosynthesis: | Â | Â | Â | Â | Â | Â | Â | Â |
Dxr | HpiD1 | HpiD1 | AmbD1 | WelD1 | WelD1 | WelD1 | WelD1 | 96.4 |
Dxs | HpiD2 | HpiD2 | AmbD2 | WelD2 | WelD2 | WelD2 | WelD2 | 97.7 |
IspG | HpiD3 | HpiD3 | AmbD3 | WelD3 | WelD3 | WelD3 | WelD3 | 98.7 |
IspH | HpiD4 | HpiD4 | AmbD4 | WelD4 | WelD4 | WelD4 | - | 95.3 |
Isonitrile biosynthesis: | Â | Â | Â | Â | Â | Â | Â | Â |
IsnA | HpiI1 | HpiI1 | AmbI1 | WelI1 | WelI1 | WelI1 | WelI1 | 94 |
IsnA | HpiI2 | HpiI2 | AmbI2 | WelI2 | WelI2 | WelI2 | WelI2 | 96.2 |
IsnB | HpiI3 | HpiI3 | AmbI3 | WelI3 | WelI3 | WelI3 | WelI3 | 95.6 |
Prenyltransferases: | Â | Â | Â | Â | Â | Â | Â | Â |
Aromatic prenyltransferase | HpiP1 | HpiP1 | AmbP1 | WelP1 | WelP1 | WelP1 | WelP1 | 96.9 |
GPP | HpiP2 | HpiP2 | AmbP2 | WelP2 | WelP2 | WelP2 | - | 93 |
Aromatic prenyltransferase | - | - | AmbP3 | - | - | - | - | - |
Methyltransferases: | Â | Â | Â | Â | Â | Â | Â | Â |
N-methyltransferase | - | - | - | WelM1 | WelM1 | WelM1 | - | 98.8 |
SAM-dependent methyltransferase | - | - | - | WelM2 | WelM2 | WelM2 | WelM2 | 91.2 |
Histamine N-methyltransferase | - | - | - | WelM3 | WelM3 | WelM3 | - | 99 |
Regulation proteins | Â | Â | Â | Â | Â | Â | Â | Â |
Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain | HpiR1 | HpiR1 | AmbR1 | WelR1 | WelR1 | WelR1 | - | 93.4 |
Transcriptional regulator, LuxR family | HpiR2 | HpiR2 | AmbR2 | WelR2 | WelR2 | WelR2 | - | 96.2 |
Response regulator with CheY-like receiver domain and winged-helix DNA-binding domain | - | - | - | WelR3 | WelR3 | WelR3 | WelR3 | 93.3 |
Other: | Â | Â | Â | Â | Â | Â | Â | Â |
Dephospho-CoA kinase-like protein | HpiC1 | HpiC1 | AmbC1 | WelC1 | WelC1 | WelC1 | WelC1 | 93.2 |
Phosphoglycerate mutase family protein | HpiC3 | HpiC3 | AmbC3 | WelC3 | WelC3 | WelC3 | WelC3 | 96.4 |
Transporter genes: | Â | Â | Â | Â | Â | Â | Â | Â |
DevC protein | - | HpiE1 | AmbE1 | - | - | - | - | 98.2 |
ABC exporter membrane fusion protein, DevB family | - | HpiE2 | AmbE2 | - | - | - | - | 99.7 |
Conserved membrane hypothetical protein | - | HpiE3 | AmbE3 | - | - | - | - | 100 |
Small multidrug resistance protein | - | - | - | WelE4 | WelE4 | WelE4 | - | 97.8 |