HPLC analyses of both cis and trans isomers of indole-isonitrile from culture extracts. HPLC was analyzed at 310 nm with a UV detector. X-axis – retention times in minutes (min). Y-axis refers to intensity in arbitrary units. Plot presented as a stacked Y-plot and is drawn to relative intensity units. A) FS ATCC43239 extracts. B) FA UTEX1903 extracts. C) Synthesized cis isomer (tR = 8.8 min). D) Synthesized trans isomer (tR = 13.1 min). Peaks show only relative intensities and are not normalized for concentration of metabolites.