Figure 3

Phylogenetic analysis of Subsection V strains using 16S rDNA. Phylogenetic tree was constructed using a 929 bp fragment of the 16S rRNA gene from Subsection V cyanobacterial strains analyzed in this study, Subsection V cyanobacterial strains for which the genome has been sequenced, and cyanobacterial strains known to produce hapalindole-type natural products. Fischerella muscicola UTEX 1829 [GenBank: AB075984], Fischerella sp. PCC 9339 [IMG Gene ID: 2517062088], Fischerella sp. ATCC 43239 [GenBank: KJ768872], Fischerella ambigua UTEX 1930 [GenBank: KJ768871], Fischerella muscicola SAG 1427-1 [GenBank: AB075985], Fischerella sp. PCC 9431 [IMG Gene ID: 2512976007], Hapalosiphon welwitschii UH strain IC-52-3 [GenBank: KJ767019], Westiella intricata UH strain HT-29-1 [GenBank: KJ767016], Hapalosiphon hibernicus BZ-3-1 [GenBank: EU151900], Fischerella sp. CENA 19 [GenBank: AY039703], Fischerella sp. JSC-11 [GenBank: HM636645], Fischerella thermalis PCC 7521 [GenBank: AB075987], Fischerella muscicola PCC 7414 [GenBank: AB075986], Chlorogloeopsis fritschii PCC 6912 [GenBank: AB093489], Chlorogloeopsis fritschii PCC 9212 [GenBank: AB075982], Fischerella sp. PCC 9605 [IMG Gene ID: 2516144612], Mastigocladopsis repens PCC 10914 [GenBank: AJ544079], Mastigocoleus testarum BC 008 [IMG Gene ID: 2264826627] and Synechocystis sp. PCC 6803 [GenBank: NR_074311]. *indicates hpi/amb/wel gene cluster was identified in these strains. ^ indicates these strains are known producers of hapalindole-family of natural products. Synechocystis sp. PCC 6803 was used as the outgroup. Phylogenetic trees were constructed using the Geneious Tree Builder program, using the neighbour-joining method. Numbers at each branch point are the bootstrap values for percentages of 100 replicate trees.