Competition assays showing the participation of mannose receptor (MR) during the association of Streptococcus pneumoniae with Schwann cells (SCs). The assays were performed by adding increasing doses of mannan (10 to 1000 μg/ml) in the interaction medium, and the results were highly statistically significant (***P <0.0001) at a dose equal to or higher than 100 μg/ml. The graph shows an inhibition of the percentage of SCs with associated bacteria immediately after 3 h of association (black bar versus white bar). However, this percentage was not significantly affected after this time up to 24 h of infection in mannan-treated cultures (black bar versus dark-gray bar). These data are representative of three separate experiments, each of which was conducted in triplicate. For statistical analysis, we used Two-way ANOVA and Tukey’s Multiple Comparison Test.