Figure 2

Effect of ClpP, RpoS and CsrA on growth in LB at 10°C. Overnight cultures were diluted 1000-fold in LB and incubated at 10°C without aeration. Growth was measured by enumeration on LB agar at 37°C. A) Growth of C5 (■, full line), clpP mutant (▲, full line) and clpP ⁺ mutant (▲, broken line). B) Growth of C5 (■, full line), clpP mutant (▲, full line) for extend period. One biological replicate are shown. C) Growth of C5 (■, full line), rpoS mutant (▲, full line), clpP/rpoS mutant (♦, full line) and clpP ⁺ /rpoS mutant (♦, broken line). D) Growth of C5 (■, full line), csrA sup mutant (▲, full line), csrA ⁺ sup mutant (▲, broken line), clpP/csrA sup (■, broken line), rpoS/csrA sup (●, broken line) and clpP/rpoS/csrA sup mutant (♦, broken line). The results are average of three independent biological replicates and SD are shown except rpoS/csrA sup and clpP/rpoS/csrA sup that were performed twice and csrA ⁺ sup that were performed once.