Table 4 List of plasmids used in this study
From: Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon
Plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
pMLBAD | Broad host range vector containing inducible promoter Pbad, Tmr | [24] |
pRW50 | Low copy-number vector containing a promoter-less lacZ gene, Tcr | [26] |
pOT182 | Source of oriT sequence, Tcr | [27] |
pK18mobsac | Conjugative, suicide vector containing sacB gene, Kmr | [52] |
pRW50mob | pRW50 containing oriT sequence from pOT182, Tcr | This study |
pMLbspR | pMLBAD containing bspR orf from KHW, Tmr | This study |
pMLbprP | pMLBAD containing bprP orf from KHW, Tmr | This study |
pMLbprQ | pMLBAD containing bprQ orf from KHW, Tmr | This study |
pMLbprPQ | pMLBAD containing bprP and bprQ orfs from KHW, Tmr | This study |
pMLbsaNs/bicA | pMLBAD containing shorter bsaN orf (GTG start) and bicA orf from KHW, Tmr | This study |
pMLbsaN | pMLBAD containing longer bsaN orf (ATG start) from KHW, Tmr | This study |
pMLbsaN/bicA | pMLBAD containing longer bsaN orf (ATG start) and bicA orf from KHW, Tmr | This study |
pRWbsaM | PbsaM-lacZ transcriptional fusion, pRW50mob containing bsaM upstream sequence from KHW, Tcr | This study |
pRWbsaN | PbsaN-lacZ transcriptional fusion, pRW50mob containing bsaN upstream sequence from KHW, Tcr | This study |
pRWbicA | PbicA-lacZ transcriptional fusion, pRW50mob containing bicA upstream sequence from KHW, Tcr | This study |
pRWbicA1 | PbicA1-lacZ transcriptional fusion, pRW50mob containing truncated bicA upstream sequence from KHW, type 1 deletion, Tcr | This study |
pRWbicA2 | PbicA2-lacZ transcriptional fusion, pRW50mob containing truncated bicA upstream sequence from KHW, type 2 deletion, Tcr | This study |
pRWbopE | PbopE-lacZ transcriptional fusion, pRW50mob containing bopE upstream sequence from KHW, Tcr | This study |
pRWbopA | PbopA-lacZ transcriptional fusion, pRW50mob containing bopA upstream sequence from KHW, Tcr | This study |
pRWbopA1 | PbopA1-lacZ transcriptional fusion, pRW50mob containing truncated bopA upstream sequence from KHW, type 1 deletion, Tcr | This study |
pRWbopA2 | PbopA2-lacZ transcriptional fusion, pRW50mob containing truncated bopA upstream sequence from KHW, type 2 deletion, Tcr | This study |
pRWbprB | PbprC-lacZ transcriptional fusion, pRW50mob containing bprB upstream sequence from KHW, Tcr | This study |
pRWbprD | PbprD-lacZ transcriptional fusion, pRW50mob containing bprD upstream sequence from KHW, Tcr | This study |
pRWbprD1 | PbprD1-lacZ transcriptional fusion, pRW50mob containing truncated bprD upstream sequence from KHW, type 1 deletion, Tcr | This study |
pRWbprD2 | PbicA2-lacZ transcriptional fusion, pRW50mob containing truncated bicA upstream sequence from KHW, type 2 deletion, Tcr | This study |
pRWbprC | PbprC-lacZ transcriptional fusion, pRW50mob containing bprC upstream sequence from KHW, Tcr | This study |
pRWs1518 | Ps1518-lacZ transcriptional fusion, pRW50 containing BPSS1518 upstream sequence from KHW, Tcr | This study |
pRWs1518a | Ps1518a-lacZ transcriptional fusion, pRW50 containing truncated BPSS1518 upstream sequence from KHW, type 1 deletion, Tcr | This study |
pRWs1518b | Ps1518b-lacZ transcriptional fusion, pRW50 containing truncated BPSS1518 upstream sequence from KHW, type 2 deletion, Tcr | This study |
pRWvirA | PvirA-lacZ transcriptional fusion, pRW50 containing virA upstream sequence from KHW, Tcr | This study |
pRWvirA1 | PvirA1-lacZ transcriptional fusion, pRW50 containing truncated virA upstream sequence from KHW, type 1 deletion, Tcr | This study |
pRWtssM | PtssM-lacZ transcriptional fusion, pRW50 containing tssM upstream sequence from KHW, Tcr | This study |