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Table 4 List of plasmids used in this study

From: Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon

Plasmid Relevant characteristic(s)a Source or reference
pMLBAD Broad host range vector containing inducible promoter Pbad, Tmr [24]
pRW50 Low copy-number vector containing a promoter-less lacZ gene, Tcr [26]
pOT182 Source of oriT sequence, Tcr [27]
pK18mobsac Conjugative, suicide vector containing sacB gene, Kmr [52]
pRW50mob pRW50 containing oriT sequence from pOT182, Tcr This study
pMLbspR pMLBAD containing bspR orf from KHW, Tmr This study
pMLbprP pMLBAD containing bprP orf from KHW, Tmr This study
pMLbprQ pMLBAD containing bprQ orf from KHW, Tmr This study
pMLbprPQ pMLBAD containing bprP and bprQ orfs from KHW, Tmr This study
pMLbsaNs/bicA pMLBAD containing shorter bsaN orf (GTG start) and bicA orf from KHW, Tmr This study
pMLbsaN pMLBAD containing longer bsaN orf (ATG start) from KHW, Tmr This study
pMLbsaN/bicA pMLBAD containing longer bsaN orf (ATG start) and bicA orf from KHW, Tmr This study
pRWbsaM PbsaM-lacZ transcriptional fusion, pRW50mob containing bsaM upstream sequence from KHW, Tcr This study
pRWbsaN PbsaN-lacZ transcriptional fusion, pRW50mob containing bsaN upstream sequence from KHW, Tcr This study
pRWbicA PbicA-lacZ transcriptional fusion, pRW50mob containing bicA upstream sequence from KHW, Tcr This study
pRWbicA1 PbicA1-lacZ transcriptional fusion, pRW50mob containing truncated bicA upstream sequence from KHW, type 1 deletion, Tcr This study
pRWbicA2 PbicA2-lacZ transcriptional fusion, pRW50mob containing truncated bicA upstream sequence from KHW, type 2 deletion, Tcr This study
pRWbopE PbopE-lacZ transcriptional fusion, pRW50mob containing bopE upstream sequence from KHW, Tcr This study
pRWbopA PbopA-lacZ transcriptional fusion, pRW50mob containing bopA upstream sequence from KHW, Tcr This study
pRWbopA1 PbopA1-lacZ transcriptional fusion, pRW50mob containing truncated bopA upstream sequence from KHW, type 1 deletion, Tcr This study
pRWbopA2 PbopA2-lacZ transcriptional fusion, pRW50mob containing truncated bopA upstream sequence from KHW, type 2 deletion, Tcr This study
pRWbprB PbprC-lacZ transcriptional fusion, pRW50mob containing bprB upstream sequence from KHW, Tcr This study
pRWbprD PbprD-lacZ transcriptional fusion, pRW50mob containing bprD upstream sequence from KHW, Tcr This study
pRWbprD1 PbprD1-lacZ transcriptional fusion, pRW50mob containing truncated bprD upstream sequence from KHW, type 1 deletion, Tcr This study
pRWbprD2 PbicA2-lacZ transcriptional fusion, pRW50mob containing truncated bicA upstream sequence from KHW, type 2 deletion, Tcr This study
pRWbprC PbprC-lacZ transcriptional fusion, pRW50mob containing bprC upstream sequence from KHW, Tcr This study
pRWs1518 Ps1518-lacZ transcriptional fusion, pRW50 containing BPSS1518 upstream sequence from KHW, Tcr This study
pRWs1518a Ps1518a-lacZ transcriptional fusion, pRW50 containing truncated BPSS1518 upstream sequence from KHW, type 1 deletion, Tcr This study
pRWs1518b Ps1518b-lacZ transcriptional fusion, pRW50 containing truncated BPSS1518 upstream sequence from KHW, type 2 deletion, Tcr This study
pRWvirA PvirA-lacZ transcriptional fusion, pRW50 containing virA upstream sequence from KHW, Tcr This study
pRWvirA1 PvirA1-lacZ transcriptional fusion, pRW50 containing truncated virA upstream sequence from KHW, type 1 deletion, Tcr This study
pRWtssM PtssM-lacZ transcriptional fusion, pRW50 containing tssM upstream sequence from KHW, Tcr This study
  1. aAbbreviations: Ap r ampicillin resistant, Km r kanamycin resistant, Tc r tetracycline resistant, Tm r trimethoprim resistant.