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Table 1 Bacterial strains and plasmids used in this study

From: Selenite reduction by the obligate aerobic bacterium Comamonas testosteroni S44 isolated from a metal-contaminated soil

Strain or plasmid Relevant properties or derivation Source or reference
C. testosteroni S44 Wild type, Rifr, Cms, Tets [26]
iscR-280, iscR-327, iscR-513 iscR Tn5 insertional mutants This study
Rifr, Cmr, Tets
iscS + 30 Tn5 insertional mutant downstream of iscR, Rifr, Cmr, Tets This study
E. coli S17-1(λpir) Tpr Smr recA thi pro hsdR hsdM +. RP4:2Tc:Mu:Km T7, λpir Lab collection
Plasmids   
pRL27-Cm Transposon vector , ori R6K , Cmr [52]
pCPP30 Broad host range, tetA Timothy R. McDermott, Montana State University