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Table 1 Bacterial strains and plasmids used in this study

From: Selenite reduction by the obligate aerobic bacterium Comamonas testosteroni S44 isolated from a metal-contaminated soil

Strain or plasmid

Relevant properties or derivation

Source or reference

C. testosteroni S44

Wild type, Rifr, Cms, Tets

[26]

iscR-280, iscR-327, iscR-513

iscR Tn5 insertional mutants

This study

Rifr, Cmr, Tets

iscS + 30

Tn5 insertional mutant downstream of iscR, Rifr, Cmr, Tets

This study

E. coli S17-1(λpir)

Tpr Smr recA thi pro hsdR − hsdM +. RP4:2Tc:Mu:Km T7, λpir

Lab collection

Plasmids

  

pRL27-Cm

Transposon vector , ori R6K , Cmr

[52]

pCPP30

Broad host range, tetA

Timothy R. McDermott, Montana State University