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Table 3 Comparison of the phylotype richness, diversity and evenness values of the isolates and 16S rRNA clones from lab-reared and field-collected A. stephensi mosquitoes.

From: Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial vector

Index

Lab-reared A. stephensi

Field-collected A. stephensi

 

Culturable

Unculturable

Culturable

Unculturable

 

M

F

M

F

M

F

L

M

F

L

No. of isolates/clones

18

16

24

24

17

34

30

85

69

66

S a

11

11

15

7

14

29

29

27

36

36

H b

1.74

1.84

2.14

1.97

2.75

2.93

3.21

2.93

3.15

3.49

E c

0.89

0.94

0.89

0.70

0.99

0.93

0.98

0.98

0.98

0.99

C_ACE

45

43

43

31

50

173

157

72

160

123

C_Chao

25

30

30

15

35

104

129

71

117

94

C_Simpson

0.013

0.011

0.08

0.54

0.017

0.02

0.02

0.11

0.11

0.06

Good's Coverage

39

32

38

71

18

15

13

69

49

46

  1. The table lists the number of phylotypes, observed and estimated species richness, coverage and diversity indices for the culturables and 16S rRNA clone libraries from lab-reared and field- collected adult and larval Anopheles stephensi mosquitoes. Numbers were calculated with DOTUR program, OTUs were defined using a distance level of 3%.
  2. The Shannon-Weiner diversity index [16] is calculated as follows:
  3. a: S = (Phylotype richness): Total number of species in the sample.
  4. b: H = Σ (pi) (log2 p - i), where p represents the proportion of a distinct phylotype relative to the sum of all phylotypes.
  5. c: E = (Evenness) was calculated as follows: E = H/Hmax where Hmax = log2 (S)
  6. C_ACE = ACE Coverage, C_Chao = Chao Coverage, C_Simpson = Simpson Coverage
  7. Good's Coverage = [1 - (n/N)] × 100
  8. Where n is the number of molecular species represented by one clone (single-clone OTUs) and N is the total number of sequences [54].
  9. M: Adult Male Anopheles stephensi
  10. F: Adult Female Anopheles stephensi
  11. L: Anopheles stephensi Larvae