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Table 2 Abundance of isolates and clones within the bacterial domain derived from the 16S rRNA gene sequences of isolates from field- collected A. stephensi.

From: Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi-an Asian malarial vector

Group

Adult Male

Culturable

Adult Male

Unculturable

Adult Female

Culturable

Adult Female

Unculturable

Larvae

Culturable

Larvae

Unculturable

 

OTUa

Matches

OTU

Matches

OTU

Matches

OTU

Matches

OUT

Matches

OTU

Matches

Cyano

-

-

-

-

 

-

 

-

-

-

1(1)

Calothrix sp.

Actino

1(1)b

Micrococcus

sp.

-

-

-

-

-

-

-

-

1(1)

Brevibacterium paucivorans

CFB

group

-

-

1(1)

Flexibacteriaceae

1(1)

Chryseobacterium indologenes

-

-

2(2)

C. indologenes

1(1)

Dysqonomonas

sp.

Firmicutes

1(1)

Staphylococcus hominis

1(1)

Bacillus sp.

-

-

1(1)

Leuconostoc citreum

1(1)

Bacillus sp.

2(2)

Staphylococcus

cohnii

 

1(1)

S. saprophyticus

6(21)

Paenibacillus alginolyticus

-

-

-

-

1(1)

B. cereus

1(1)

S. suis

 

-

-

1(1)

P. chondroitinus

-

-

-

-

1(1)

B. firmus

3(5)

B. thermo

amylovorans

 

-

-

7(31)

Paenibacillaceae

-

-

-

-

3(3)

Exiguo

bacterium

1(1)

Lactobacillus

Beta-Proteo bacteria

-

-

1(1)

Herbaspirillum sp.

-

-

1(1)

Achromobacter xylosoxidans

-

-

3(5)

Azoarcus sp.

 

-

-

-

-

-

-

-

-

-

-

1(1)

Leptothrix sp.

 

-

-

-

-

 

-

 

-

-

-

1(1)

Hydroxenophaga

Gamma-Proteo bacteria

2(2)

Acinetobacter

1(1)

Photorhabdus luminescens

1(2)

Acinetobacter

2(4)

Acinetobacter

5(6)

A. venetianus

1(1)

Enterobacter aerogenes

 

1(2)

A. lwofii

-

-

1(1)

A. hemolyticus

2(3)

A. hemolyticus

1(1)

Aeromonas

sobria

1(1)

Ignatzschineria larvae sp.

 

3(3)

A. radioresistens

-

-

3(4)

A. radioresistens

1(1)

Acinetobacter

sp.

1(1)

A. popoffii

1(1)

Enterobacter

sp.

 

1(2)

A. johnsonii

-

-

1(1)

Citrobacter freundii

2(2)

Pseudomonas putida

4(4)

P. anquilliseptica

2(6)

Serratia

sp.

 

1(1)

Enterobacter

-

-

4(6)

Enterobacter

2(2)

P. synxantha

1(1)

Pseudo

xanthomonas

1(1)

Serratia

sp.

 

1(2)

E. cloacae

-

-

14(15)

E. cloacae

1(1)

Pseudomonas

sp.

4(4)

Thorsellia anopheles

2(3)

T. anopheles

 

-

-

-

-

2(2)

E. sakazaki

8(23)

S. marcescens

2(2)

Vibrio chlorae

6(24)

S. marcescens

 

2(2)

Escherichia hermani

-

-

1(1)

E. hermani

6(15)

S. nematodiphila

-

-

4(6)

S. nematodiphila

 

-

-

-

-

-

-

1(1)

S. proteamaculans

-

-

-

-

 

-

-

-

-

-

-

1(1)

Xenorhabdus nematodiphila

-

-

-

-

 

-

-

-

-

-

-

1(1)

Leminorella grimontii

-

-

-

-

 

-

-

-

-

-

-

2(4)

Uncultured

-

-

-

-

 

-

-

-

-

1(1)

Entero

bacteriaceae

1(1)

Entero

bacteriaceae

-

-

-

-

Deinococcus

-

-

-

-

-

-

-

-

1(1)

Deinococcus xinjiangensis

2(4)

D. xinjiangensis

Uncultured

-

-

9(28)

Uncultured

-

-

4(8)

Uncultured

2(2)

Uncultured

1(1)

Uncultured

No match

3

No matchc

15

No match

2

No match

10

No match

7

No match

1

No match

Total

14

(17)

Species = 10

27

(85)

Species = 8

29

(34)

Species = 10

36

(69)

Species = 16

29

(30)

Species = 14

36

(66)

Species = 20

  1. Distribution of the clones and OTUs in taxonomic groups and their abundance in the individual samples are displayed. a: Operational Taxonomic Units, b: Values in parenthesis corresponds to total number of microbial strains identified, c: No significant similarity found (Sequences not included for analysis).
  2. Total number of phylotypes observed: Field-collected adult male A. stephensi = 41,
  3. Field-collected adult female A. stephensi = 65, Field-collected larvae of A. stephensi = 65.