Northern blot analyses of CcpA-dependent genes. A, Transcription of genes showing differential expression in the ccpA mutant in the absence of glucose. Gene expression at an OD600 of 1 in strain Newman and its ΔccpA mutant is shown. B, Transcription of CcpA-dependent, glucose-dependent genes in strain Newman and its ΔccpA mutant. Cells were grown to an OD600 of 1, cultures where split and glucose added to one half (+), while the other half remained without glucose (-). RNA was sampled at an OD600 of 1, and after 30 min. RNA loading is represented by the intensity of the 16S rRNA. Data are representative for at least two independent experiments. MA, microarray data.