Figure 2

Northern blot analyses of CcpA-dependent genes. A, Transcription of genes showing differential expression in the ccpA mutant in the absence of glucose. Gene expression at an OD₆₀₀ of 1 in strain Newman and its ΔccpA mutant is shown. B, Transcription of CcpA-dependent, glucose-dependent genes in strain Newman and its ΔccpA mutant. Cells were grown to an OD₆₀₀ of 1, cultures where split and glucose added to one half (+), while the other half remained without glucose (-). RNA was sampled at an OD₆₀₀ of 1, and after 30 min. RNA loading is represented by the intensity of the 16S rRNA. Data are representative for at least two independent experiments. MA, microarray data.