A. Neighbour-joining tree of 19 kDa homologs. Family members are found in both slow-growing and fast-growing mycobacteria and in the closely related genera, Nocardia and Rhodococcus. The predicted 19 kDa proteins fall into three sub-families: LpqH, LppE and Lp3. B. Nucleotide sequence of the N-terminal coding sequence of the 19 kDa gene indicating the sequences that were modified in the Δ19 strains complemented by the non-acylated or non-O-glycosylated 19 kDa molecule. The disruption to sequence encoding the N-Acyl diglyceride motif is indicated by underlined text and the disruption of the 2 threonine clusters shown in bold. The protein sequence of the wild type and each variant is also shown. Amino acid numbering is based upon the mature protein after cleavage of the signal peptide.