Table 2 DNA Primers used
Target DNA | Primer name | Sequence (5'-3') | Amplicon size |
|---|---|---|---|
manAO-Ag | manA-A | CATCACCATCGTTCAGAGCA | 1804 bp |
manA-B | GCCAGGGGAAATGATAATGA | ||
manBO-Ag | manB-A | GTTGGCAGAAGTTGGCATCG | 2091 bp |
manB-B | CTAATGCCTGTTCCGCCACC | ||
manCO-Ag | manC-A | TTGAAGACTGGTTTATTGCG | 2210 bp |
manC-B | GCAAGACTGCCATAGAAACC | ||
wbkE | wbkE-A | CCGCAAACTGAATGGATAAA | 2452 bp |
wbkE-B | GCAACTGTCAGGTCTGGTGC | ||
wbkF | wecA-A | GCGGAGGAATGGACAAGGAC | 1234 bp |
wecA-B | AGGAAAGCCTGGCGGTACTG | ||
wbkD | wbkD-A | TGGCTGGAGTGTGCCGAAAG | 2165 bp |
wbkD-B | ACGGTTGCTGGTGCTTGTGG | ||
wa** | wa**-A | CATCACGCATAATGACACCG | 2342 bp |
wa**-B | TGCTTTTGACAAGCTCGTCG | ||
manBcore | manBcore-A | CCAGCCGACGATTGAACTGG | 1589 bp |
manBcore-B | AAGCCTTGAACCCGATCCCC | ||
wboA | wboA-A | TCTGCATAACGGTCCTTGCC | 1334 bp |
wboA-B | GCTTTTACGGCAACAAGTCC | ||
wboB | wboB-A | CTTGGGATGCGAAACTACCG | 1656 bp |
wboB-B | GCTCACGCTTCCGAATACTG |