Figure 10

Motuporamines inhibit the degradation of internalized FITC-EGF and causes intracellular accumulation of EGFR. (A) Cells labelled with FITC-EGF at 4°C were exposed to DMSO (control) or 5 μM analogue 20 (motuporamine) for 0, 30 min or 6 h at 37°C, and FITC-EGF was visualized by fluorescence microscopy. (B) Cells were exposed to DMSO (control) or 5 μM analogue 20 for 24 h at 37°C, and EGFR was visualized by immunofluorescence microscopy.