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Table 4 Substrate specificities of the AHL-acylase Aac against AHLs

From: A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity

  AHL-acylase activities (nmol·min-1·ml-1)a
AHLs E. coli DH10B (pBBR1MCS-3) E. coli DH10B
(pS3aac)
C4-HSL 0.26 ± 0.15 (7.2%) 0.37 ± 0.13 (10.3%)
C6-HSL 0.31 ± 0.15 (8.7%) 0.38 ± 0.10 (10.5%)
3OC6-HSL 0.37 ± 0.09 (10.5%) 0.35 ± 0.09 (10.5%)
C7-HSL 0.23 ± 0.15 (6.4%) 3.60 ± 0.31 (100.0%)*
C8-HSL 0.21 ± 0.16 (5.7%) 1.63 ± 0.21 (45.4%)*
3OC8-HSL 0.22 ± 0.17 (6.1%) 2.56 ± 0.04 (71.1%)*
C10-HSL 0.25 ± 0.15 (7.1%) 3.10 ± 0.25 (86.1%)*
  1. a Values represent the averages ± standard errors from triplicate independent HSL-OPA assays. The relative activity in parentheses is based on defining C7-HSL-degrading activity exhibited by E. coli DH10B (pS3aac) as 100%. The value with an asterisk (*) is the significant difference from the E. coli DH10B (pBBR1MCS-3) shown in the same row by one-tailed Student's t-test (P < 0.05)