A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity

Table 3 The minimal inhibitory concentrations of aculeacin A for Candida tropicalis F-129

	OD₆₀₀^a at serial diluted aculeacin A (μg·ml^-1)
Aculeacin A	0	0.001	0.005	0.01	0.05	0.1	0.5	1
Untreated	0.592 ± 0.036*	0.615 ± 0.088*	0.255 ± 0.096*	0.126 ± 0.029*	0.045 ± 0.006	0.047 ± 0.008	0.043 ± 0.002	0.041 ± 0.004
Aac treated	0.629 ± 0.032*	0.634 ± 0.047*	0.297 ± 0.030*	0.093 ± 0.017*	0.070 ± 0.035	0.054 ± 0.007	0.044 ± 0.002	0.042 ± 0.005

^a Values represent the averages ± standard errors from triplicate independent assays. By one-tailed student's t-test (P < 0.05), the value with an asterisk (*) designates a significant difference from the 1 μg·ml^-1 of aculeacin A test shown in the same row. There is no significant difference between Aac-untreated aculeacin A and Aac-treated aculeacin A in the same column

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