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Table 2 The AHL-degrading abilities of E. coli DH10B (pS3aac) evaluated by whole cell bioassay

From: A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity

 

AHL-degrading abilitiesa

 

C6-HSL

C7-HSL

C8-HSL

Test strains

IPTG(+)

IPTG(-)

IPTG(+)

IPTG(-)

IPTG(+)

IPTG(-)

 

C

S

C

S

C

S

C

S

C

S

C

S

E. coli DH10B (pBBR1MCS-3)

-

-

-

-

-

-

-

-

-

-

-

-

E. coli DH10B (pS3aac)

-

-

-

-

+

-

+

-

+

-

+

-

  1. a The whole cells (C) and cell-free supernatants (S) of test strains, were prepared from the 0.4 mM IPTG induced and uninduced cultures, and then reacted with various AHLs to perform whole cell bioassays. Identical to the result in Fig. 1, the absence of a violet lawn indicates a positive AHL-degrading ability and is defined as'+'; a violet lawn indicates no AHL-degrading ability and is defined as'-'
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BMC Microbiology

ISSN: 1471-2180

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