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Table 2 The AHL-degrading abilities of E. coli DH10B (pS3aac) evaluated by whole cell bioassay

From: A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity

  AHL-degrading abilitiesa
  C6-HSL C7-HSL C8-HSL
Test strains IPTG(+) IPTG(-) IPTG(+) IPTG(-) IPTG(+) IPTG(-)
  C S C S C S C S C S C S
E. coli DH10B (pBBR1MCS-3) - - - - - - - - - - - -
E. coli DH10B (pS3aac) - - - - + - + - + - + -
  1. a The whole cells (C) and cell-free supernatants (S) of test strains, were prepared from the 0.4 mM IPTG induced and uninduced cultures, and then reacted with various AHLs to perform whole cell bioassays. Identical to the result in Fig. 1, the absence of a violet lawn indicates a positive AHL-degrading ability and is defined as'+'; a violet lawn indicates no AHL-degrading ability and is defined as'-'