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Figure 4 | BMC Microbiology

Figure 4

From: A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity

Figure 4

The effects of Aac on the production of violacein and chitinase activity in C. violaceum CV026. The plasmids pBBR1MCS-3 and pS3aac were transformed into C. violaceum CV026. Both of them were cultivated in LB containing tetracycline as well as 25 μM C7-HSL. (a) Cell growth was monitored by measuring the OD600. (b) The violacein production was determined by OD576 during growth. The data represent the mean values of three independent experiments. (c) The overnight cultures of C. violaceum CV026 (pS3aac) and C. violaceum CV026 (pBBR1MCS-3) (no aac insert) were seeded onto an LA plate containing tetracycline, C7-HSL and chitin in order to assay the chitinolytic activity. The plates were incubated at 30°C for 5 d. The formation of a clear zone around the colonies indicated positive chitinolytic activity.

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