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Figure 3 | BMC Microbiology

Figure 3

From: A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity

Figure 3

ESI-MS spectrometry analysis of C7-HSL degradation by AHL-acylase Aac. The E. coli DH10B (pS3aac) cells were suspended in 0.1 mM sodium phosphate and 0.01 mM ammonia acetate, respectively, and then mixed with 25 μM C7-HSL for the degradation reaction described in Materials and Methods. (a) To detect HSL, the ESI+-MS spectra of undigested C7-HSL (top) and degraded C7-HSL products (bottom) were shown. (b) To detect heptanoic acid, the ESI--MS spectra of undigested C7-HSL (top) and degraded C7-HSL products (bottom) were shown. (c) Mechanism of C7-AHL degradation by Aac. The white arrow indicates the Aac catalytic site.

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