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Figure 1 | BMC Microbiology

Figure 1

From: A probable aculeacin A acylase from the Ralstonia solanacearum GMI1000 is N-acyl-homoserine lactone acylase with quorum-quenching activity

Figure 1

The Aac acylase degrades C7-HSL in C. violaceum CV026 cultures and inhibits violacein production. The E. coli DH10B (pS3aac) overnight culture was centrifuged, and the harvested cells were suspended into 100 mM Tris buffer (pH 7.0). The cell suspensions and cell free supernatants were mixed with 25 μM C7-HSL each and then incubated at 30°C for 1 h. The mixtures were assayed by the in vitro whole cell bioassay. Well 1, C7-HSL (AHL-non-degrading control); well 2, Tris buffer (AHL-degrading control); well 3, the mixture of cell suspensions with C7-HSL; well 4, the mixture of supernatants with C7-HSL.

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