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Table 4 Detailed results from the external validation study.

From: Validation of a same-day real-time PCR method for screening of meat and carcass swabs for Salmonella

Salmonella serotype Inoculation level (cfu/25 g) Real-time PCRa Salmonella BAX
Detection System
   Ct-value for Salmonella Ct-value for IAC Final result Final result
Infantis 1000 20.05 27.89 Positive Positive
  100 21.66 29.09 Positive Positive
  10 27.14 28.68 Positive Positive
  10 30.59 28.95 Positive Positive
  10 24.92 28.89 Positive Positive
  5 29.42 29.09 Positive Positive
  5 26.57 28.81 Positive Positive
  5 26.29 27.66 Positive Positive
  5 26.63 28.79 Positive Positive
  2 27.70 28.42 Positive Positive
  2 25.68 28.08 Positive Positive
  2 27.86 28.56 Positive Positive
  2 27.20 28.90 Positive Positive
Agona 1000 22.47 28.97 Positive Positive
  100 24.70 27.93 Positive Positive
  10 > 36 29.21 Negative Negative
  10 > 36 29.07 Negative Negative
  10 26.04 28.93 Positive Positive
  5 28.47 28.76 Positive Positive
  5 32.93 28.53 Positive Negative
  5 29.84 28.92 Positive Positive
  5 32.17 27.90 Positive Positive
  2 > 36 28.76 Negative Positive
  2 > 36 29.07 Negative Negative
  2 33.22 28.77 Positive Positive
  2 30.61 27.96 Positive Positive
Infantis 1000 19.59 29.01 Positive Positive
  100 23.74 28.86 Positive Positive
  10 25.55 28.45 Positive Positive
  10 24.85 28.40 Positive Positive
  10 26.82 28.36 Positive Positive
  5 29.82 29.10 Positive Positive
  5 29.03 28.16 Positive Positive
  5 24.77 28.28 Positive Positive
  5 > 36 > 40 Inconclusive Positive
  2 28.61 27.88 Positive Positive
  2 26.24 28.79 Positive Positive
  2 26.02 28.82 Positive Positive
  2 > 36 28.63 Negative Negative
  1. Results from 39 pork meat samples inoculated with salmonella at different levels and analyzed in parallel on-site using the real-time PCR and the Salmonella BAX methods.
  2. a Samples with a Ct value > 36 is considered negative if the Ct value for the IAC is < 40 and inconclusive if a Ct > 40 is obtained for the IAC. According to the Method Directive for the PCR method, re-analysis of the extracted DNA by PCR is then needed.