Table 4 Primer information for real-time qPCR.
Gene name | Size (bp) | Forward primer sequence (5' – 3') | Reverse primer sequence (5' – 3') |
|---|---|---|---|
PT3-inducible genes | |||
PCNA | 66 | GAACTGGTTCATTCATCTCTATGG | TGTCACAGACAAGTAATGTCGATAAA |
POLD1 | 107 | AGGTAGTACTGCGTGTCAATGG | CCCTACGTGATCATCAGTGC |
RFC3 | 69 | CACAGTAGATAACACGTGGCAAA | AGTAGGTGCTTGGCGGTTC |
RFC4 | 77 | TTCCAGGTGGTCCGTAAAAC | AGAAGTGGTTGCAGTGCTGA |
RFC5 | 84 | TGTGGCCGGTATTTTTCAAC | GGAGACCTCAGCACTCAAGC |
RPA1 | 65 | CCGTAGTAATGGGACGGATG | GCAGAAGGGGGATACAAACA |
RPA2 | 95 | TTCCAGAATATGTGTGGTGAACTC | GCCACCTGAGATCTTTTCAGA |
Reference genes | |||
ACTB | 97 | CCAGAGGCGTACAGGGATAG | CCAACCGCGAGAAGATGA |
GAPDH | 66 | GCCCAATACGACCAAATCC | AGCCACATCGCTCAGACAC |