Figure 3

Real-time quantitative PCR analysis (open bars) of genes selected from the microarray (closed bars) in PT3 and Non-PT3. Data are expressed relative to ACTB (3A) and GAPDH (3B) mRNA and (*) presented p < 0.05. The gene expression levels were sorted by detector. Gene expression levels for PT3 are indicated by the black bar. This color also indicates the sample in the RQ sample grid and the RQ results panel plots. Because NK samples are used as calibrator, the expression levels are set to 1. But because the gene expression levels were plotted as log₁₀ values (and the log₁₀ of 1 is 0), the expression level of the calibrator samples appear as 0 in the graph. In addition, because the relative quantities as the targets are normalized against the relative quantities of the reference genes, the expression level of the reference genes is 0, that is, there are no bars for ACTB and GAPDH. Fold-expression changes were calculated using the equation 2^-ΔΔCT [5]. Error bars for each column in the plot provided that the associated expression level was calculated from 3 replicates. The error bars display the calculated maximum (RQMax) expression levels that represent standard error of the mean expression level (RQ value). Collectively, the upper and lower limits defined the region of expression within which the true expression level value was likely to occur. The error bars was based on the RQMin/Max confidence level. The number associated with each bar indicates the linear fold-change of mRNA expression in PT3 relative to Non-PT3 for comparison.