High AAV replication and virion production in PT3 cells. Equal numbers of the indicated cells were infected with AAV, cultured in the organotypic epithelial raft system and analyzed for AAV DNA replication and virion production as described in the materials and methods section. A shows the experimental scheme. B shows the resulting level of AAV DNA replication in a "first plate" experiment done in triplcate. Note that PT3 allows for the highest level of AAV DNA replication. C shows a densitometric quantification of the experiment shown in B. D shows the resulting level of AAV DNA replication in a "first plate" experiment, similar to that done in B, however the monomer duplex (md) and single stranded (ss) bands are not as overexposed as in B. E shows the level of AAV virion production by infection and replication in a "second plate" of adenovirus-infected 293 cells. Again, note that PT3 allows for the highest level of AAV virion production.