Schematic representation of the major metabolic pathways that are up- or down-regulated by heat stress at 48°C. The three letter designations for the enzymes involved in the heat stress response can be found in the KEGG web site for S. aureus N135 http://www.genome.jp/kegg/. When there are several genes within the same operon that are increased, then the three letter designation is followed by capital letters, which represents the different enzymes (genes). When the gene/enzyme is not definitively known, then the gene number in the N315 ordered sequence tag numbering is given, e.g., SA1336 for glucose-6-phosphate 1-dehydrogenase. Arrows before the enzymes indicate significant increases (upward arrow) or decreases (downward arrow) in the transcripts or the key metabolic product that is produced by the pathway. All of the steps in the metabolic pathways are not shown, rather just key branch-points, so as to simplify the figure. Several important changes are shown at the bottom of the slide that do not fit into the central metabolism of the cell.