Recruitment of Rac and Arf6 to C. caviae GPIC entry sites. HeLa cells transfected with Rac-GFP (A) or Arf6-HA (B) for 24 h were infected with C. caviae GPIC in the presence of DMSO (control) or 60 μM INP0341. At 10 minutes p.i. cells were fixed and processed for immunofluorescence. Top panels show labelling for the small GTPases, middle panels show actin labelling and bottom panels show superimposition of the two images, as well as host cell nuclei stained with Hoechst 33342 (blue). Rac and Arf6 are recruited to sites of actin polymerization (arrows), both in control cells and in cells treated with INP0341.