Figure 4
Differential Interference Contrast (DIC) microscopy (left) and fluorescence microscopy with DAPI (right) of C. albicans (isolate 77) control and treated with MIC 50 of AZA and EIL, showing alterations in the cell cycle such as the presence of cells with multiple nuclei (arrows in Fig. D and G), abnormal chromatin condensation (arrowheads in Fig. E and H), and cells without a nucleus (asterisk in Fig. F and I). A-C: control cells in different stages of the cell cycle; D-F: 0.25 μg.ml-1 AZA; G-I: 1 μg.ml-1 EIL; J: Percentage of C. albicans cells, untreated and treated with 24-SMT inhibitors, showing different cell cycle stages: (I) cells with no bud and one nucleus, (II) cells with a bud and one nucleus, and (III) cells with a bud and two nuclei (one in each cell); and alterations of cell cycles: (A) cells with more than one nucleus, (B) cells showing abnormal chromatin condensation, and (C) cells without a nucleus. Bar = 5 μm.